The table below shows the material flows in m 3 h around the ion exchange column.
Ion exchange column diagram.
Key steps in the ion exchange chromatography procedure are listed below.
Thus the workflow below is given as a generalized iex workflow and particular running conditions for cation exchange chromatography may be adjusted to best suit your protein of interest the buffer system and the anion.
Ion exchange ix systems are used across a variety of industries for water softening purification and separation purposes.
The hardness ions ca and mg move into the resin beads and each of these divalent.
Aside from its use to purify drinking water the technique is widely applied for purification and separation of a variety of industrially and medicinally.
An impure protein sample is loaded into the ion exchange chromatography column at a particular ph.
The interstitial liquid fed with the resin contains caustic soda and the liquor flow corresponds to one bed volume.
Ion exchange chromatography can be applied to the determination of ionic solutes such as inorganic anions cations transition metals and low molecular weight organic acids.
Usage examples continuous ion exchangers are used when it is imperative that product be continually generated.
Ion exchange usually describes a processes of purification of aqueous solutions using solid polymeric ion exchange resin more precisely the term encompasses a large variety of processes where ions are exchanged between two electrolytes.
While the chemistry of individual ion exchange reactions varies from one application to the next ix is a treatment process where dissolved ions are replaced by other more desirable ions of a similar electrical charge.
Ion exchange chromatography workflow.
Ion exchange takes place in one column while the resin in the second column is being regenerated resulting in continuous operation.
Rohm and haas ion exchange ion exchange introduction 4 fd sep 2008 to soften water you take a cation exchange resin on which the mobile ion inside the beads is sodium na and you pass the hard water through a column filled with the sodium form resin.
Schematic diagram of the principles of chromatography as discovered by tswett 1901.
After loading an impure protein sample onto an ion exchange chromatography column the column is washed to remove undesired proteins and other impurities and then the protein s of interest is eluted using either a salt gradient or a change in ph.
Ion exchange dealkalization systems produce hardness free low alkalinity water at a reasonable cost and with a high degree of reliability.
For example metal contaminants are undesirable due to their color taste odor or toxicity.
Split stream and weak acid cation systems also reduce the total dissolved solids.
The ion exchange column has a solid resin feed of 10 m 3 h and an interstitial liquid factor of 0 85.
Cation exchange chromatography workflow.
